Monday 6 April 2015

Proteins important for pathogenesis - HWp1

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Candida albicans
Candida albicans 2.jpg
Scientific classification
Kingdom:Fungi
Division:Ascomycota
Class:Saccharomycetes
Order:Saccharomycetales
Family:Saccharomycetaceae
Genus:Candida
Species:C. albicans
Binomial name
Candida albicans
(C.P.Robin) Berkhout (1923)
Synonyms
  • Candida stellatoidea[1]
  • Oidium albicans[2]
Candida albicans is a diploid fungus that grows both as yeast and filamentous cells and a causal agent of opportunistic oral and genital infections in humans,[3][4] and candidal onychomycosis, an infection of the nail plate. Systemic fungal infections (fungemias) including those by C. albicans have emerged as important causes of morbidity and mortality in immunocompromised patients (e.g., AIDS, cancer chemotherapy, organ or bone marrow transplantation).[citation needed] C. albicans biofilms may form on the surface of implantable medical devices. In addition, hospital-acquired infections by C. albicans have become a cause of major health concerns.
C. albicans is commensal and a constituent of the normal gut flora comprising microorganisms that live in the human mouth and gastrointestinal tract. C. albicans lives in 80% of the human population without causing harmful effects, although overgrowth of the fungus results in candidiasis (candidosis). Candidiasis is often observed in immunocompromised individuals such as HIV-infected patients. A common form of candidiasis restricted to the mucosal membranes in mouth or vagina is thrush, which is usually easily cured in people who are not immunocompromised. For example, higher prevalence of colonization of C. albicans was reported in young individuals with tongue piercing, in comparison to unpierced matched individuals.[5] To infect host tissue, the usual unicellular yeast-like form of C. albicans reacts to environmental cues and switches into an invasive, multicellular filamentous form, a phenomenon called dimorphism.[3]


Genome[edit]

Candida albicans growing on Sabouraud agar
One of the most important features of the C. albicans genome is the occurrence of numeric and structural chromosomal rearrangements as means of generating genetic diversity, named chromosome length polymorphisms (contraction/expansion of repeats), reciprocal translocations, chromosome deletions and trisomy of individual chromosomes. These karyotypic alterations lead to changes in the phenotype, which is an adaptation strategy of this fungus. These mechanisms will be better understood with the complete analysis of the C. albicans genome.
An unusual feature of the Candida genus is that in many of its species (including C. albicans and C. tropicalis, but not, for instance, C. glabrata) the CUG codon, which normally specifies leucine, specifies serine in these species. This is an unusual example of a departure from the standard genetic code, and most such departures are in start codons or, for eukaryotes, mitochondrial genetic codes.[6][7][8] This alteration may, in some environments, help these Candida species by inducing a permanent stress response, a more generalized form of the heat shock response.[9]
The genome of C. albicans is highly dynamic, and this variability has been used advantageously for molecular epidemiological studies and population studies in this species. The genome sequence has allowed for identifying the presence of a parasexual cycle (no detected meiotic division) in C. albicans.[10] This study of the evolution of sexual reproduction in six Candida species found recent losses in components of the major meiotic crossover-formation pathway, but retention of a minor pathway.[10] The authors suggested that if Candida species undergo meiosis it is with reduced machinery, or different machinery, and indicated that unrecognized meiotic cycles may exist in many species. In another evolutionary study, introduction of partial CUG identity redefinition (from Candida species) into Saccharomyces cerevisiae clones caused a stress response that negatively affected sexual reproduction. This CUG identity redefintion, occurring in ancestors of Candida species, was thought to lock these species into a diploid or polyploid state with possible blockage of sexual reproduction.[11]

Dimorphism[edit]

Although often referred to as "dimorphic", C. albicans is in fact polyphenic. When cultured in standard yeast laboratory medium, C. albicans grows as ovoid "yeast" cells. However, mild environmental changes in temperature and pH can result in a morphological shift to pseudohyphal growth.[12] Pseudohyphae share many similarities with yeast cells,[13] but their role during candidiasis remains unknown. When C. albicans cells are grown in a medium that mimics the physiological environment of a human host, they grow as "true" hyphae. Its ability to form hyphae has been proposed as a virulence factor, as these structures are often observed invading tissue, and strains that are unable to form hyphae are defective in causing infection. Candida albicans can also form Chlamydospores, the function of which remains unknown.[14]
Round, white-phase and elongated, opaque-phase Candida albicans cells: the scale bar is 5 µm.
In this model of the genetic network regulating the white-opaque switch, the white and gold boxes represent genes enriched in the white and opaque states, respectively. The blue lines represent relationships based on genetic epistasis. Red lines represent Wor1 control of each gene, based on Wor1 enrichment in chromatin immunoprecipitation experiments. Activation (arrowhead) and repression (bar) are inferred based on white- and opaque-state expression of each gene.
In a process that superficially resembles dimorphism, C. albicans undergoes a process called phenotypic switching, in which different cellular morphologies are generated spontaneously. Of the classically studied strains, one that undergoes phenotypic switching is WO-1,[15] which consists of two phases: one that grows as round cells in smooth, white colonies and one that is rod-like and grows as flat, gray colonies. The other strain known to undergo switching is 3153A; this strain produces at least seven different colony morphologies. In both the WO-1 and 3153A strains, the different phases convert spontaneously to the other(s) at a low frequency. The switching is reversible, and colony type can be inherited from one generation to another. While several genes that are expressed differently in different colony morphologies have been identified, some recent efforts focus on what might control these changes. Further, whether a potential molecular link between dimorphism and phenotypic switching occurs is a tantalizing question.[16]
In the 3153A strain, a gene called SIR2 (for silent information regulator), which seems to be important for phenotypic switching, has been found. SIR2 was originally found in Saccharomyces cerevisiae (brewer's yeast), where it is involved in chromosomal silencing—a form of transcriptional regulation, in which regions of the genome are reversibly inactivated by changes in chromatin structure (chromatin is the complex of DNA and proteins that make chromosomes). In yeast, genes involved in the control of mating type are found in these silent regions, and SIR2 represses their expression by maintaining a silent-competent chromatin structure in this region. The discovery of a C. albicans SIR2 implicated in phenotypic switching suggests it, too, has silent regions controlled by SIR2, in which the phenotype-specific genes may reside.
Another potential regulatory molecule is Efg1p, a transcription factor found in the WO-1 strain that regulates dimorphism, and more recently has been suggested to help regulate phenotypic switching. Efg1p is expressed only in the white and not in the gray cell-type, and overexpression of Efg1p in the gray form causes a rapid conversion to the white form.[17][18]
So far, very few data suggest dimorphism and phenotypic switching use common molecular components. However, it is not inconceivable that phenotypic switching may occur in response to some change in the environment, as well as being a spontaneous event. How SIR2 itself is regulated in S. cerevisiae may yet provide clues as to the switching mechanisms of C. albicans.

Heterozygosity[edit]

The heterozygosity of the Candida genome exceeds that found in other genomes and is widespread among clinical isolates. Nonsynonymous single-base polymorphisms result in two proteins that differ in one or several amino acids that may confer functional differences for each protein. This situation considerably increases the number of different proteins encoded by the genome.[19]

Proteins important for pathogenesis[edit]

Hwp1[edit]

Hwp1 stands for Hyphal Wall protein 1. Hwp1 is a mannoprotein located on the surface of the hyphae in the hyphal form of Candida albicans. Hwp1 is a mammalian transglutaminase substrate. This host enzyme allows Candida albicans to attach stably to host epithelial cells.[20] Adhesion of Candida albicans to host cells is an essential first step in the infection process for colonization and subsequent induction of mucosal disease.

Slr1[edit]

RNA-binding protein Slr1 was recently discovered to play a role in instigating the hyphal formation and virulence in C. albicans.[21]

Application in engineering[edit]

Candida albicans has been used in combination with carbon nanotubes (CNT) to produce stable electrically conductive bio-nano-composite tissue materials that have been used as temperature sensing elements[22]

Treatment[edit]

Treatment commonly includes:[23]

See also[edit]

Portal iconFungi portal

References[edit]

  1. ^ Candida albicans at NCBI Taxonomy browser, url accessed 2006-12-26
  2. ^ "McClary, Dan Otho (May 1952). "Factors Affecting the Morphology of Candida Albicans". Annals of the Missouri Botanical Garden 39 (2): 137–164. doi:10.2307/2394509. JSTOR 2394509. 
  3. ^ a b Ryan KJ, Ray CG (editors) (2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. ISBN 0-8385-8529-9. 
  4. ^ dEnfert C; Hube B (editors) (2007). Candida: Comparative and Functional Genomics. Caister Academic Press. ISBN 978-1-904455-13-4. 
  5. ^ Zadik Yehuda, Burnstein Saar, Derazne Estella, Sandler Vadim, Ianculovici Clariel, Halperin Tamar (March 2010). "Colonization of Candida: prevalence among tongue-pierced and non-pierced immunocompetent adults". Oral Dis 16 (2): 172–5. doi:10.1111/j.1601-0825.2009.01618.x. PMID 19732353. 
  6. ^ Ohama, T; Suzuki, Tsutomu; Mori, Miki; Osawa, Syozo; Ueda, Takuya; Watanabe, Kimitsuna; Nakase, Takashi (August 1993). "Non-universal decoding of the leucine codon CUG in several Candida species". Nucleic Acids Research 21 (17): 1039–4045. doi:10.1093/nar/21.17.4039. PMC 309997. PMID 8371978. 
  7. ^ Arnaud, MB; Costanzo, MC; Inglis, DO; Skrzypek, MS; Binkley, J; Shah, P; Binkley, G; Miyasato, SR; Sherlock, G. "CGD Help: Non-standard Genetic Codes". Candida Genome Database. Retrieved 30 October 2011. 
  8. ^ Andrzej (Anjay) Elzanowski and Jim Ostell (7 July 2010). "The Alternative Yeast Nuclear Code". The Genetic Codes. Bethesda, Maryland, U.S.A.: National Center for Biotechnology Information (NCBI). Retrieved 30 October 2011. 
  9. ^ Santos, MA; Cheesman, C; Costa, V; Moradas-Ferreira, P; Tuite, MF (February 1999). "Selective advantages created by codon ambiguity allowed for the evolution of an alternative genetic code in Candida spp.". Molecular Microbiology 31 (3): 937–947. doi:10.1046/j.1365-2958.1999.01233.x. PMID 10048036. 
  10. ^ a b Butler G, Rasmussen MD, Lin MF, et al. (June 2009). "Evolution of pathogenicity and sexual reproduction in eight Candida genomes". Nature 459 (7247): 657–62. doi:10.1038/nature08064. PMC 2834264. PMID 19465905. 
  11. ^ Silva RM, Paredes JA, Moura GR, et al. (October 2007). "Critical roles for a genetic code alteration in the evolution of the genus Candida". EMBO J. 26 (21): 4555–65. doi:10.1038/sj.emboj.7601876. PMC 2063480. PMID 17932489. 
  12. ^ Peter E. Sudbery (2011). "Growth of Candida albicans hyphae". Nature Reviews Microbiology 9 (10): 737–748. doi:10.1038/nrmicro2636. PMID 21844880.  See figure 2.
  13. ^ Berman J, Sudbery PE (2002). "Candida Albicans: a molecular revolution built on lessons from budding yeast". Nature Reviews Genetics 3 (12): 918–930. doi:10.1038/nrg948. PMID 12459722. 
  14. ^ Staib P, Morschhäuser J (2007). "Chlamydospore formation in Candida albicans and Candida dubliniensis--an enigmatic developmental programme.". Mycoses 50 (1): 1–12. doi:10.1111/j.1439-0507.2006.01308.x. PMID 17302741. 
  15. ^ Rikkerrink E, Magee B, Magee P (1988). "Opaque-white phenotype transition: a programmed morphological transition in Candida albicans". J. Bact. 170 (2): 895–899. PMC 210739. PMID 2828333. 
  16. ^ Soll D.R. (2012). Signal Transduction Pathways Regulating Switching, Mating and Biofilm Formation in Candida Albicans and Related Species. In: G. Witzany (ed). Biocommunication of Fungi. Springer, 85-102. ISBN 978-94-007-4263-5.
  17. ^ Sonneborn A, Tebarth B, Ernst J (1999). "Control of white-opaque phenotypic switching in Candida albicans by the Efg1p morphogenetic regulator". Infection and Immunity 67 (9): 4655–4660. PMC 96790. PMID 10456912. 
  18. ^ Srikantha T, Tsai L, Daniels K, Soll D (2000). "EFG1 Null Mutants of Candida albicans Switch but Cannot Express the Complete Phenotype of White-Phase Budding Cells". J. Bact. 182 (6): 1580–1591. doi:10.1128/JB.182.6.1580-1591.2000. PMC 94455. PMID 10692363. 
  19. ^ Larriba G; Calderone RA (2008). "Heterozygosity and Loss of Heterozygosity in Candida albicans". Pathogenic Fungi: Insights in Molecular Biology. Caister Academic Press. ISBN 978-1-904455-32-5. 
  20. ^ Staab, J. F. (1999). "Adhesive and Mammalian Transglutaminase Substrate Properties of Candida albicans Hwp1". Science 283 (5407): 1535–1538. doi:10.1126/science.283.5407.1535. ISSN 0036-8075. 
  21. ^ Ariyachet, C.; Solis, N. V.; Liu, Y.; Prasadarao, N. V.; Filler, S. G.; McBride, A. E. (2013). "SR-like RNA-binding protein Slr1 affects Candida albicans filamentation and virulence". Infection and Immunity 81 (4): 1267–1276. doi:10.1128/IAI.00864-12. ISSN 0019-9567. 
  22. ^ Di Giacomo, Raffaele; Maresca, Bruno; Porta, Amalia; Sabatino, Paolo; Carapella, Giovanni; Neitzert, Heinz-Christoph (2013). "Candida albicans/MWCNTs: A Stable Conductive Bio-Nanocomposite and Its Temperature-Sensing Properties". IEEE Transactions on Nanotechnology 12 (2): 111. doi:10.1109/TNANO.2013.2239308. 
  23. ^ Rambach, G; Oberhauser, H; Speth, C; Lass-Flörl, C (2011). "Susceptibility of Candida species and various moulds to antimycotic drugs: Use of epidemiological cutoff values according to EUCAST and CLSI in an 8-year survey". Medical mycology : official publication of the International Society for Human and Animal Mycology 49 (8): 856–63. doi:10.3109/13693786.2011.583943. PMID 21619497. 
  24. ^ Lingappa, BT; Prasad, M; Lingappa, Y; Hunt, DF; Biemann, K (1969). "Phenethyl alcohol and tryptophol: Autoantibiotics produced by the fungus Candida albicans". Science 163 (3863): 192–4. doi:10.1126/science.163.3863.192. PMID 5762768. 

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